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Genotoxicity and cytotoxicity of zinc oxide and titanium dioxide in HEp-2 cells

Osman, Ilham F, Baumgartner, Adi ORCID: https://orcid.org/0000-0001-7042-0308, Cemeli, Eduardo, Fletcher, Jonathan N and Anderson, Diana (2010) Genotoxicity and cytotoxicity of zinc oxide and titanium dioxide in HEp-2 cells. Nanomedicine, 5 (8). pp. 1193-1203.

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Osman et al 2010 final draft manuscript.pdf_sequence=3&isAllowed=y - Accepted Version


Aims: The rapidly growing industrial and medical use of nanomaterials, especially zinc oxide and titanium dioxide, has led to growing concerns about their toxicity. Accordingly, the intrinsic genotoxic and cytotoxic potential of these nanoparticles have been evaluated. Materials & methods: Using a HEp-2 cell line, cytotoxicity was tested along with mitochondrial activity and neutral red uptake assays. The genotoxic potential was determined using the Comet and the cytokinesis-blocked micronucleus assays. In addition, tyrosine phosphorylation events were investigated. Results & conclusion: We found concentration- and time-dependent cytotoxicity and an increase in DNA and cytogenetic damage with increasing nanoparticle concentrations. Mainly for zinc oxide, genotoxicity was clearly associated with an increase in tyrosine phosphorylation. Our results suggest that both types of nanoparticles can be genotoxic over a range of concentrations without being cytotoxic.

Item Type: Article
Status: Published
DOI: https://doi.org/10.2217/nnm.10.52
Subjects: R Medicine > RZ Other systems of medicine
School/Department: School of Science, Technology and Health
URI: https://ray.yorksj.ac.uk/id/eprint/6051

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