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Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay.

Kavanagh, Owen, Estes, Mary K, Reeck, Amanda, Raju, Ravikiran M, Opekun, Antone R, Gilger, Mark A, Graham, David Y and Atmar, Robert L (2011) Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay. Clinical and vaccine immunology : CVI, 18 (7). pp. 1187-90.

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Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Item Type: Article
Status: Published
DOI: https://doi.org/10.1128/CVI.00039-11
Subjects: Q Science > QR Microbiology > QR180 Immunology
Q Science > QR Microbiology > QR355 Virology
School/Department: School of Health Sciences
URI: http://ray.yorksj.ac.uk/id/eprint/866

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