Weier, JF, Ferlatte, C, Baumgartner, A ORCID: https://orcid.org/0000-0001-7042-0308, Nguyen, HN, Weier, BA and Weier, HUG (2023) Analysis of human invasive cytotrophoblasts demonstrates mosaic aneuploidy. PLOS ONE.

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Abstract

A total of 24 chromosome-specific fluorescence in situ hybridization probes for interphase nucleus analysis were developed to determine the chromosomal content of single interphase cells. Individual human invasive cytotrophoblasts derived from in vitro cultured assays were fully karyotyped by repeated interphase fluorescence in situ hybridization studies. At least 75% of invasive cytotrophoblasts were hyperdiploid and the total number of chromosomes ranged from 47 to 61. The results also demonstrated that these hyperdiploid invasive cytotrophoblasts showed significant heterogeneity. The most copy number gains were observed for chromosomes 13, 14, 15, 19, 21, and 22 with average copy number greater than 2.3. A parallel study using primary invasive cytotrophoblasts also showed a similar trend of copy number changes. Conclusively, 24-chromosome analysis of human non-proliferating cytotrophoblasts (interphase nuclei) was achieved. Hyperdiploidy and chromosomal heterogeneity without endoduplication in invasive cytotrophoblasts may suggest a selective advantage for invasion and short lifespan during normal placental development.

Item Type:	Article
Status:	Published
DOI:	10.1371/journal.pone.0284317
Subjects:	R Medicine > RG Gynecology and obstetrics R Medicine > RZ Other systems of medicine
School/Department:	School of Science, Technology and Health
URI:	https://ray.yorksj.ac.uk/id/eprint/6328

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