Peng, D., Kavanagh, Owen ORCID: https://orcid.org/0000-0002-2599-8511, Gao, H., Zhang, X., Deng, S., Chen, D., Liu, Z., Xie, C., Situ, C. and Yuan, Z. (2020) Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues. Food Chemistry, 302.

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Abstract

To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.

Item Type:	Article
Status:	Published
DOI:	10.1016/ j.foodchem.2019.04.022
Subjects:	Q Science > Q Science (General) S Agriculture > S Agriculture (General) T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
School/Department:	School of Science, Technology and Health
URI:	https://ray.yorksj.ac.uk/id/eprint/3811

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