Quick Search:

Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues

Peng, D., Kavanagh, Owen ORCID: https://orcid.org/0000-0002-2599-8511, Gao, H., Zhang, X., Deng, S., Chen, D., Liu, Z., Xie, C., Situ, C. and Yuan, Z. (2020) Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues. Food Chemistry, 302.

[img] Text (PDF)
MQCA in swine tissues Food Chem 2019.pdf - Accepted Version
Restricted to Repository staff only until 6 April 2020.
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Abstract

To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.

Item Type: Article
Status: Published
DOI: https://doi.org/10.1016/ j.foodchem.2019.04.022
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General)
T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
School/Department: School of Health Sciences
URI: http://ray.yorksj.ac.uk/id/eprint/3811

University Staff: Request a correction | RaY Editors: Update this record